We show that, for example, mutations in CTNNB1/Ctnnb1 (human/mouse gene) do not always lead to upregulation of expression of downstream pathway targets (GLUL/Glul, LGR5/Lgr5, LECT2/Lect2 or NOTUM/Notum) in human or mouse HCC (Extended Data Fig. 4b–f). This evidence concerns the gene NOTUM and hepatocellular carcinoma.