NES and central nervous system cancer: Immunocytochemistry for neuronal (neurofilament) and glioma (nestin) markers, together with a presynaptic marker (synapsin 1) and GABRG2–GFP revealed colocalization of neuronal presynaptic puncta with glioma postsynaptic GABRG2–GFP puncta by confocal microscopy (Fig. 2b,c, Extended Data Fig. 4a and Supplementary Video 1).