GPHN and central nervous system cancer: Quantifying synaptic, colocalized gephyrin puncta and non-colocalized, extrasynaptic gephyrin puncta on DMG glioma cells following confocal microscopy and 3D reconstruction, we found that approximately 20% of glioma cell gephyrin puncta are colocalized with presynaptic VGAT (Extended Data Fig. 4b).