Here, we used lineage tracing technique to explore the lineage reprogramming of hepatocytes in SBT-induced ICC, in which hepatocytes were not only transformed into ICC cells, but also transformed into tdTomato+α-SMA+ and tdTomato+CD133+ cells, suggesting that SBT induces hepatocytes to evolve to the multifunctional cells. This evidence concerns the gene PROM1 and intrahepatic cholangiocarcinoma.