MANF and intrahepatic cholangiocarcinoma: To investigate the effect of MANF on hepatocyte transformation into ICC cells, we crossed MANFfl/fl mice with tdTomatofl/fl mice and then injected AAV8-TBG-Cre via tail vein to construct mature hepatocyte-specific MANF KI/KO lineage tracing mice (Supplementary Fig. 7).