The interactions between endogenous FASN and these hotspot mutp53 were observed by co-IP assays in different human cancer cell lines expressing homozygous endogenous mutp53, including breast cancer SK-BR3 (R175H) and MDA-MB468 (R273H), as well as colorectal cancer LS1034 (G245S) cells (Fig. 1b). The gene discussed is FASN; the disease is breast carcinoma.