To investigate MEOX2 and GLI-1 roles in lung tumor progression, we conducted a genetic silencing experiment (shRNAs) targeting these genes using A549 and NCI-H1975 NSCLC cells and analyzed mRNA expression and protein levels of the EGFR oncogene, as well as key epigenetic markers from the Trithorax group, including CBP and SMARCB1, and EZH2, a member of the Polycomb repressive complex. This evidence concerns the gene MEOX2 and non-small cell lung carcinoma.