High-dimensional spatial maps and cellular interaction analysis also demonstrated closer proximity between PMN-MDSCs and tumor cells, CD4+ T cells, and CD8+ T cells in FGFR2 iCCA as compared to other subsets, providing additional evidence that PMN-MDSCs are a major contributor to induce local immune suppression in FGFR2 iCCA. This evidence concerns the gene CD8A and neoplasm.