In addition to the above-mentioned genes, mRNAs directly associated with heart development, numerous cardiac pathologies, endothelial dysfunction, and atherosclerosis, e.g. alpha kinase 3 (ALPK3),32 junctional cadherin 5 associated,33 adrenoreceptor beta 1,34 were also enriched in the myosin-5b compared with the IgG pulldown (Figure 9E; Supplementary data online, Table S5). This evidence concerns the gene ALPK3 and atherosclerosis.