In order to confirm the regulation between RIP140, a transcriptional corepressor that plays an important role in energy metabolism, and the mitochondrial protein SIRT5 during the development of cardiac hypertrophy, we next measured the cell surface area and activation of fetal gene expression to evaluate the effect of SIRT5 on RIP140-induced cellular hypertrophy response in NRCMs. The gene discussed is SIRT5; the disease is cardiac hypertrophy.