MAP1LC3B and glioblastoma: U251 and T98G cells were transfected with mCherry-GFP-LC3B, vortioxetine increased the percentage of GBM cells displaying red and green puncta compared to untreated cells, and vortioxetine induced the formation of autophagosomes, which could be illustrated by yellow puncta, while red puncta signify autolysosomes (Figure 3A).