Our study reveals that the reduction of HSP90B1 in human 293T cells leads to increased ROS production, JNK activation, and apoptosis, while transgenic expression of HSP90B1 inhibits these processes in Drosophila. Additionally, we observed a decrease in Gp93 mRNA levels in aging fly brains, suggesting that human HSP90B1 may serve as a biomarker for the early stages of neurodegenerative diseases. This evidence concerns the gene HSP90B1 and neurodegenerative disease.