The results of the cytological experiments indicated that elevated expression of AKR1C1, CYGB, and HSF1, contrasting with reduced expression levels of IFNA10, IFNA21, CDO1, BRD2, ATF2, ATG16L1, HDDC3, DAZAP1, BCAT2, FTH1, HNRNPA2B1, ATG3, AKT1S1, DHODH, and FADS2 in the MYCN-amplified NB cell line SK-N-BE2 in comparison to the non-MYCN amplified SH-SY5Y cell line (Fig. 11A). The gene discussed is AKT1S1; the disease is neuroblastoma.