The results indicated that, in comparison with the low-risk group, the mRNA expression levels of AKR1C1, BCAT2, HNRNPA2B1, ATG3, AKT1S1, CYGB, and FADS2 were significantly elevated in the high-risk group of NB; the mRNA expression levels of IFNA10, IFNA21, CDO1, ATF2, and ATG16L were significantly decreased in the high-risk group of NB; there were no statistically significant differences in the expression levels of BRD2, HDDC3, DAZAP1, FTH1, BRD4, DHODH, and HSF1 between the two groups of NB (Supplementary Fig. 1). The gene discussed is IFNA21; the disease is neuroblastoma.