We then analyzed CALU, KIF1B, and POLR3G expression in TCGA database and NPC microarray GSE53819, the results showed that CALU and POLR3G are highly expressed in HNSC tumor tissues while KIF1B is not significant (Fig. S5A), but all of them are highly expressed in NPC tissues in GSE53819 (Fig. S5B). This evidence concerns the gene CALU and nasopharyngeal carcinoma.