3D clinostat may primarily affect immune and endocrine-related signaling pathways, such as those associated with human diseases like Malaria, African trypanosomiasis, Autoimmune thyroid disease, Rheumatoid arthritis, and Staphylococcus aureus infection, as well as GnRH secretion and signaling pathway, Ovarian steroidogenesis, IL17 signaling pathway, Prolactin signaling pathway, Leukocyte transendothelial migration, PPAR signaling pathway, and Neutrophil extracellular trap formation. The gene discussed is PPARA; the disease is human African trypanosomiasis.