H&E staining of pancreatic tissue sections showed that both mice strains developed AP within 8 h of caerulein treatment, but histologic scoring of 24 h sections detected an increased damage in the Cst3−/− mice (Fig. 7A), especially the number of necrotic cells and the number of infiltrating inflammatory cells were increased (Supplementary Fig. 13a–c). The gene discussed is CST3; the disease is alkaline phosphatase measurement.