Additionally, treatment of NCI-H358 and LS180 cancer cell lines with a previously reported SMG1-specific small molecule inhibitor, SMG1i-11, resulted in specific increases in the transcripts and proteins from genes with truncating mutations, just as did LY3023414 (Figure 3—figure supplements 2 and 3; Gopalsamy et al., 2012). Here, SMG1 is linked to cancer.