Furthermore,the successful customizable nanoprobe fabrication, as confirmed bySERS results, provide insights into molecular interactions betweenthe nanoprobes and tau and significant enhancement in signal intensity,highlighting the potential of the developed AuNP/Aptamer bioconjugatesfor SERS-based detection and analysis of protein interactions at thenanoscale, contributing to the advancement of diagnostic tools forMD, such as AD. The gene discussed is MAPT; the disease is Alzheimer disease.