In addition to the characteristic morphological appearance on histology, common immunohistochemical (IHC) analysis used for the diagnostic work-up for GCT include CD117/KIT (germinoma), POU5F1/OCT4 (germinoma), Placental alkaline phosphate (PLAP) (germinoma), AFP (yolk sac tumor), CD30 (embryonal carcinoma), and HCG (choriocarcinoma or syncytiotrophoblast in germinoma). This evidence concerns the gene AFP and yolk sac tumor.