In an in vitro PDAC tumor setting, Cas9 + hdr treated Panc-1 cells resulted in decreased M2 TAM polarization, increased M1 TAM polarization, PD-L1 depletion inhibited M2 IL-4 secretion, and upregulated M1 TNF-αsecretion. The gene discussed is GATA3; the disease is neoplasm.