To determine the functional importance of CYLD-regulated NF-κB and STAT1 activation for the control of S. aureus, we stimulated THP-1 macrophages with IFN-γ/LPS for M1 polarization and treated the cells prior to infection with the IKK VII inhibitor to inhibit activation of NF-κB and with STAT1 siRNA to inhibit STAT1. Here, CYLD is linked to infection.