MAP1LC3B and infection: To provide additional evidence that mLST8 alters autophagy flux, human undifferentiated ARPE19 cells were infected with the mLST8‐AAV2 construct followed by overnight infection with adenovirus‐GFP‐RFP‐LC3B, a pH sensitive tandem reporter construct, to label autophagosomes (yellow) and autolysosomes (red) (Valapala et al. 2014; Gupta et al. 2022).