This analysis revealed that certain salient features originate from tumor-adjacent areas, which is consistent with the findings of Austin et al. Austin et al.‘s research demonstrated that 83% of PD-L1 expression in HNSCC tumors is peripheral, with this staining pattern suggesting an induced response associated with inflammation, potentially the most sensitive to anti-PD-1 therapy [33]. The gene discussed is CD274; the disease is head and neck squamous cell carcinoma.