Aggressive, overexpressed pediatric tumors (e.g., PAX3-FOXO1 fusion-positive alveolar rhabdomyosarcoma) (Fig. 2), which are distinguished from the milder variant (embryonal rhabdomyosarcomas) provide a good example.87,88 Embryonal rhabdomyosarcomas do not harbor overexpression (under-expression) scenarios, nor does it display high mutation burden. The gene discussed is PAX3; the disease is alveolar rhabdomyosarcoma.