Subsequently, western blot assays confirmed these findings, showing that treatment with IHCH9033 led to significant downregulation of the DNA damage sensor ATM [37, 38], along with its downstream DNA repair signals, including a marked decrease in p53 phosphorylation and increased γH2AX levels in FLT3-ITD AML cells (Fig. 1k). This evidence concerns the gene TP53 and acute myeloid leukemia.