Moreover, IHCH9033 significantly enhanced AcH3 level as well as DNA damage and cell apoptosis, as indicated by elevated γH2AX levels and caspase 3 cleavage (Fig. 3i), and it also reduced the phosphorylation of STAT5, ERK and AKT (Fig. 3j) in these FLT3-ITD AML patient samples. This evidence concerns the gene AKT1 and acute myeloid leukemia.