After transfecting siRNAs targeting the top four transcription factors into HCC cells, western blotting and RT-qPCR analyses revealed that ZNF384 knockdown had the most significant impact on NOL9 expression (Fig. 4A and Supplementary Fig. 3A), suggesting that ZNF384 upregulates NOL9 in HCC cells. Here, ZNF384 is linked to hepatocellular carcinoma.