Using chromogenic multiplex IHC, for Iba1, GFAP and 4G8 (n = 5 CTL, 8 AD), we quantified the number of PaM and PaM-associated astrocytes (PaM astrocytes), those polarised around PaM and forming a net, associated with Aβ plaques (n = 391) in the CA1 and measured the corresponding plaque area (Fig. 3A–C). This evidence concerns the gene GFAP and Alzheimer disease.