CDK2 and neoplasm: Cell cycle-regulated phosphoproteins such as CDK1 and CDK2 are highly regulated and rapidly dephosphorylated and were likely thus missed in the human samples; however, substrates of AKT1 and PRKACA, which were highly phosphorylated in DMG tumor tissues, also exhibited high phosphorylation levels in the DMG cell lines, validating our tumor findings.