Cell cycle-regulated phosphoproteins such as CDK1 and CDK2 are highly regulated and rapidly dephosphorylated and were likely thus missed in the human samples; however, substrates of AKT1 and PRKACA, which were highly phosphorylated in DMG tumor tissues, also exhibited high phosphorylation levels in the DMG cell lines, validating our tumor findings. Here, PRKACA is linked to neoplasm.