PGK1 and cancer: In line with these observations, lactate dehydrogenase A (LDHA) expression, which usually increases in cancer cells opting for glycolytic metabolism, was significantly reduced following Spry1 loss along with the mRNA levels of other glycolysis-related genes, such as aldolase C (ALDOC), hexokinase II (HK2), phosphoglycerate kinase 1 (PGK1), and the glucose transporter protein type 1 (GLUT1), which facilitates the transport of glucose into cells (Fig. 3H and I).