We illustrate the resulting AlphaFold-Metainference approach for a set of well-studied highly disordered proteins30  as well as for proteins that include both ordered and disordered domains, including TAR DNA-binding protein 43 (TDP-43), which is associated in amytrophic lateral sclerosis (ALS)31, ataxin-3, which is linked with Machado–Joseph disease (also known as spinocerebellar ataxia type 3)32, and the human prion protein33, which causes Creutzfeldt–Jakob disease and related prion diseases34. Here, TARDBP is linked to amyotrophic lateral sclerosis.