BGN and osteoarthritis: For instance, the biglycan neoepitope BGN262 (the cleavage site is 262GLGHNQIRM) was shown to have a distinct cytoplasmic and intranuclear staining location in osteoarthritic subchondral bone sclerosis (44, 45) and this intracellular expression was more pronounced in osteoarthritis with increasing severity (44, 45).