Due to the longtime induction of mouse GC models and the complexity of replicating human GC in vivo, and the limitation of the unspecific Sox9‐cre or Olfm4‐cre genetic tracing in vivo, alternative genetic strategies are needed to specifically label the trans‐differentiation of gastric CSCs in vivo, such as the dual recombinase‐mediated intersectional genetic strategies using combined Dre‐rox and Cre‐loxP system. Here, SOX9 is linked to gastric cancer.