To better understand the tumor microenvironment in liver cancer, we analyzed TME cell interactions in single-cell data, focusing on the four primary immune-related groups: B, D, M, and T. The interaction dynamics within the TME (Supplementary Figure S3D) showed that the T group exhibited strong T cell interactions, particularly involving CD8+ and CD4+ T cells, promoting an active immune response. This evidence concerns the gene CD8A and liver cancer.