We further treated PC3M‐IE8 PCa cells with two PLK1 inhibitors, BI2536 (an ATP‐competitive inhibitor that dual targets PLK1 and BRD4) and Poloxin (a non‐ATP‐competitive inhibitor that targets the PLK1 PBD structural domain), followed by nocodazole blockade and Co‐IP combined with western blotting. Here, PLK1 is linked to posterior cortical atrophy.