In both glioblastoma cell lines, untreated cells exhibited a low number of 53BP1 and γH2AX foci per nucleus, 2.5 53BP1 foci/cell and 0.4 γH2AX foci/cell for U343 MG and 2 53BP1foci/cell and 0.3 γH2AX foci/cell for U87 MG (Figure 4, Supplementary Figures 3A–F). The gene discussed is TP53BP1; the disease is glioblastoma.