However, a caveat against its long-term use, in particular in the kidney stone prone CF patient population, may come from a study by Xia et al. (2014), who studied the fluid absorptive and HCO3− secretion rates in anesthetised Slc26a6-, Slc26a3, NHE3-and NHE2-deleted mice by single-pass perfusion. This evidence concerns the gene SLC26A6 and cystic fibrosis.