To investigate whether manipulating PROX1 can prevent liver tumor formation, we used a HCC mouse model combining Myc OE and Trp53 KO (Myc/Trp53) via hydrodynamic tail-vein injection (HDTVI; Fig. 2h, Extended Data Fig. 3a,b and Supplementary Table 6). Here, PROX1 is linked to hepatocellular carcinoma.