In line with observations made for the CRC TUMs (Fig. 2B), the paucimannosidic proteins identified in the LIM2405 cells were predominantly of lysosomal origin (KEGG pathway, Benjamini FDR = 2.0−9), including ASAH1, CTSB, CTSC, CTSD, MAN2B1, PPT1, PSAP, and TPP1 (Fig. 3H), five of which showed an M31850-induced reduction in paucimannosylation across seven sites (Fig. 3I). Here, CTSC is linked to colorectal carcinoma.