Metformin can mainly inhibit GPX4 protein expression in glioma cells, promote the expression of Long-chain acyl-CoA synthetase 4 (ACSL-4) protein, which leads to the increase of Reactive Oxygen Species(ROS) level, triggers ferroptosis, and inhibits glioma proliferation (20–23). The gene discussed is ACSL4; the disease is central nervous system cancer.