Moreover, rutaecarpine promotes endothelial nitric oxide synthase (eNOS) phosphorylation and NO synthesis via the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and calmodulin-dependent protein kinase kinase β (CaMKKβ)/AMP-activated protein kinase (AMPK) signaling pathways through transient receptor potential vanilloid type 1 (TRPV1), and effectively prevent endothelial dysfunction (Lee et al., 2021). The gene discussed is NOS3; the disease is endothelial dysfunction.