The top 20 signaling pathways closely related to co targets were obtained through KEGG co enrichment analysis, and it was found that the most relevant signaling pathways for insomnia were arranged in order of importance, including the neuroactive ligand receptor interaction signaling pathway, calcium signaling pathway, IL-17 signaling pathway, TNF signaling pathway, Toll like receptor signaling pathway, serotonergic synapse, etc. In Figure 13. This evidence concerns the gene TNF and insomnia.