In order to evaluate the specificity of the lateral flow aptasensor for AFP detection, a series of high concentrations of proteins including bovine serum albumin (BSA), prostate cancer antigen (PSA), carcinoembryonic antigen (CEA), ovarian cancer-associated antigen (CA-125), breast cancer-associated antigen (CA15-3), and immunoglobulin G (IgG) were selected for validation. Here, AFP is linked to breast cancer.