Furthermore, the data from the functional enrichment analysis showed an upregulation of the autophagy pathway, signified by the increased expression of cargo receptor SQSTM1 and ATG8 family proteins (GABARAP and GABARAPL1) (Figure 2d), suggesting that PAK4 KO may increase MHC I expression via the regulation of autophagy, as a recent study indicated a potential connection between pancreatic cancer cell autophagy and cell surface MHC I expression [19]. This evidence concerns the gene SQSTM1 and familial pancreatic carcinoma.