Therefore, MΦ differentiation of human THP-1 monocyte cells cultured with conditioned media obtained from cancer cell lines was analyzed as illustrated in Figure 1A. Conditioned media from indicated cancer cell lines, including lung, pancreatic, glioblastoma cell lines, and PMA induced robust THP-1 differentiation, as evidenced by CD14 expression (Figure 1B left and right panels, and Figure S1A). This evidence concerns the gene CD14 and cancer.