As shown in Figure 3A (right), among all of our engineered mutants in HNSCC background, RAC1-A159V-expressing cells displayed the highest EHop-016 sensitivity in 3D cultures, with an IC50 of 1537.2 nM, which was 2.95 times more sensitive to EGFP-control cells (IC50 = 4535.3 nM; n = 18 cumulative data from three independent experiments; p = 0.0102). This evidence concerns the gene RAC1 and head and neck squamous cell carcinoma.