Finally, the autoimmune profile should be thoroughly assessed in all patients with particular attention to the testing for MSA/MAAs in the presence of ANA positivity with a nucleolar or cytoplasmic pattern, positivity for anti-Ro52kD and/or clinical features suggestive of IIM, even if classic autoimmunity tests (such as ANA and ENA) are negative. This evidence concerns the gene ENAH and Autoimmunity.