From these clusters, five major cell types were identified via classical cell markers, including T cells (CD3E, CD8A, CD3D), B cells (CD19, MS4A1, CD79A), myeloid cells (CD14, CD68, LYZ), endothelial cells (PECAM1, VWF, CDH5) and cancer-associated fibroblasts (CAFs) (ACTA2, FAP, PDGFRB) (Figure 2C). The gene discussed is ACTA2; the disease is cancer.