This study aimed to investigate in PBMCs the active DNA demethylation process in relation to MASLD-associated fibrosis risk by analyzing both global levels of the demethylation intermediates 5hmC and 5fC and exploring the methylation profiles and expression of suppressor of cytokine signaling 3 (SOCS3), sterol regulatory element binding transcription factor 1 (SREBF1), and thioredoxin interacting protein (TXNIP), key genes involved in inflammatory processes and liver fibrosis [14,15,16,17,18,19,20]. Here, SREBF1 is linked to metabolic dysfunction-associated steatotic liver disease.