Our findings show a significant association between the AA genotype and the GG genotype for rs1426654 of the SLC24A5 gene with melasma (OR = 0.03571; 95% CI: 0.005866–0.3303; p = 0.0010), suggesting that the GG genotype may confer a higher risk for melasma development in women of African ancestry. Here, SLC24A5 is linked to freckles.