To further investigate the role of G3BP1 protein in viral infection, we overexpressed G3BP1 in PK15 cells infected with PCV2, and the results are shown in Figure 2A–D; compared to the control group, both relative expression levels of Cap and Rep and virus copy numbers, as well as protein levels, indicate a promoting effect of G3BP1 on PCV2 replication. This evidence concerns the gene G3BP1 and viral infectious disease.