The five proteins that were most significantly increased in the CRC cell line-derived EVs (Caco-2, HT29, and HCT116) were GANAB (FC = 5.4), BCAM (FC = 4.0), HIST1H4A (FC = 2.9), SERPINA1 (FC = 4.2), and FAT1 (FC = 4.2) (listed in order of decreasing significance). Here, FAT1 is linked to colorectal carcinoma.