NLRP3 and neoplasm: In order to understand the impact of the PTX-enriched GF-based scaffolds on breast cancer pathogenesis and NLRP3 inflammasome mechanism, we performed qRT-PCR to investigate the variation in the expression of the TP53, BRCA1, NLRP3, and caspase-1 genes in MDA-MB-231 tumor cells compared with normal cells (Figure 7).